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Image Search Results
Journal: Cell
Article Title: Regulation of Energy Expenditure by Brainstem GABA Neurons
doi: 10.1016/j.cell.2019.05.048
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Genotypes/sources for mice used in the above studies are listed in the . table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-GFP Abcam ab13970; RRID: AB_300798 Rabbit polyclonal anti-c-Fos Synaptic Systems 226003; RRID: AB_2231974 Rabbit monoclonal anti-c-Fos Cell Signaling 2250S; RRID: AB_2247211 Rabbit polyclonal anti-RFP Rockland 600-401-379; RRID: AB_2209751 Rat monoclonal anti-mCherry (16D7) ThermoFisher {"type":"entrez-nucleotide","attrs":{"text":"M11217","term_id":"333925"}} M11217 ; RRID: AB_2536611 Chicken polyclonal anti-mCherry Abcam ab205402; RRID: AB_2722769 Chemicals, Peptides, and Recombinant Proteins Clozapine N-oxide Sigma-Aldrich C0832-5MG Critical Commercial Assays RNAscope Multiplex Fluorescent Assay V2 Advanced Cell Diagnostics (ACD Bio) 323100 RNAscope Probe- Mm-Slc32a1 ACD Bio 319191-C1 RNAscope Probe- mCherry-C2 ACD Bio 431201-C2 RNAscope Probe- EGFP ACD Bio 400281-C1 RNAscope Probe- tdTomato-C2 ACD Bio 317041-C2 Experimental Models: Organisms/Strains Mouse: Vgat-IRES-Cre: Slc32a1 tm2(cre)Lowl /J The Jackson Laboratory 016962; RRID: ISMR_JAX:016962 Mouse: Rosa26-LSL-EGFPL10a: B6.129S4- Gt(ROSA)26Sor tm1(CAG-EGFP/Rp/10a,birA)Wtp /J The Jackson Laboratory 022367; RRID: IMSR_JAX:022367 Mouse: C57BL/6J The Jackson Laboratory 000664; RRID: IMSR_JAX:000664 Mouse: TRAP2: Fos tm2.1(icre/ERT2)Luo /J The Jackson Laboratory 030323; RRID: IMSR_JAX:030323 Mouse: Ai14: B6;129S6- Gt(ROSA)26Sor tm14(CAG-tdTomato)/Hze /J The Jackson Laboratory 007908; RRID: IMSR_JAX:007908 Recombinant DNA AAV5-EF1a-DIO-hChR2(H134R)-EYFP UNC Vector Core N/A AAV5-EF1a-DIO-EYFP UNC Vector Core N/A AAV5-hSyn-DIO-hM3D(Gq)-mCherry Addgene 44361 AAV5-hSyn-DIO-hM4D(Gi)-mCherry Addgene 44362 AAV5-EF1a-fDIO-HA-NLS-Cre This paper; available at Addgene N/A AAV9-CAG-FLEX-EGFP; AI854 Penn Vector Core N/A AAV9-DIO-SynP-mCherry MGH Vector Core AAV-RN1 AAV9-DIO-SynP-EYFP MGH Vector Core AAV-RN2 PRV-lp298 (PRV-mCherry) This paper N/A PRV-152 (PRV-GFP) Smith et al., 2000 N/A Software and Algorithms GraphPad Prism 6.0 GraphPad N/A ClearMap Renier et al., 2016 N/A Imaris 9.0.1 Bitplane N/A Terastitcher University Campus Bio-Medico of Rome N/A Open in a separate window KEY RESOURCES TABLE METHOD DETAILS Viral Vectors All viruses used in these studies were obtained from UNC Vector Core, Penn Vector Core,
Techniques: Recombinant, Multiplex Assay, Fluorescence, Plasmid Preparation, Software